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PCR applications = protocols for functional genomics /

Record Type:	Electronic resources : Monograph/item
Title/Author:	PCR applications/ edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.
Reminder of title:	protocols for functional genomics /
other author:	Sninsky, John J.
Published:	San Diego :Academic Press, : c1999.,
Description:	1 online resource (xviii, 566 p., [3] p. of plates) :ill. (some col.) :
Subject:	Polymerase chain reaction - Diagnostic use. -
Online resource:	http://www.sciencedirect.com/science/book/9780123721853
ISBN:	9780123721853

PCR applications = protocols for functional genomics /
PCR applicationsprotocols for functional genomics /[electronic resource] :edited by Michael A. Innis, David H. Gelfand, John J. Sninsky. - San Diego :Academic Press,c1999. - 1 online resource (xviii, 566 p., [3] p. of plates) :ill. (some col.)

Includes bibliographical references and index.

pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity -- Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR -- Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research -- Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome -- Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval.

ISBN: 9780123721853

Source: 78885:78885Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

125008
Polymerase chain reaction
--Diagnostic use.Index Terms--Genre/Form:

96803
Electronic books.

LC Class. No.: QP606.D46 / P346 1999eb

Dewey Class. No.: 572.8/6

National Library of Medicine Call No.: 1999 E-525

PCR applications = protocols for functional genomics /
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300 $a 1 online resource (xviii, 566 p., [3] p. of plates) : $b ill. (some col.)
504 $a Includes bibliographical references and index.
505 0 $a pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity -- Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR -- Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research -- Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome -- Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.
520 $a PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval.
588 $a Description based on print version record.
650 4 $a Polymerase chain reaction $x Diagnostic use. $3 125008
650 6 $a Reaction en chaine de la polymerase. $3 256305
650 6 $a Amplification genique. $3 256306
650 6 $a Reaction en chaine de la polymerase $x Manuels de laboratoire. $3 256307
650 6 $a Amplification genique $v Manuels de laboratoire. $3 256308
650 1 7 $a Polymerase kettingreactie. $2 gtt $3 256309
650 1 7 $a Genoom. $2 gtt $3 256310
650 0 $a Polymerase chain reaction. $3 256302
650 0 $a Gene amplification. $3 256303
650 0 $a Polymerase chain reaction $v Laboratory manuals. $3 125007 $3 176375
650 0 $a Gene amplification $v Laboratory manuals. $3 256304
650 0 $a Genomics $v Congresses. $3 127037 $3 176508
650 1 2 $a Polymerase Chain Reaction $x methods. $3 72395
650 2 2 $a Genetic Engineering. $3 82107
650 4 $a Genomes. $3 245796
655 4 $a Electronic books. $2 local. $3 96803
700 1 $a Sninsky, John J. $3 256299
700 1 $a Innis, Michael A. $3 256300
700 1 $a Gelfand, David H. $3 256301
776 0 8 $i Print version: $t PCR applications. $d San Diego : Academic Press, c1999 $z 0123721857 $z 9780123721853 $w (DLC) 99211203 $w (OCoLC)41339460
856 4 0 $3 ScienceDirect $u http://www.sciencedirect.com/science/book/9780123721853
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