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Molecular imaging = FRET microscopy and spectroscopy /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular imaging/ edited by Ammasi Periasamy, Richard N. Day.
Reminder of title:	FRET microscopy and spectroscopy /
other author:	Periasamy, Ammasi.
Published:	Oxford ;Published for the American Physiological Society by Oxford University Press, : 2005.,
Description:	xv, 312 p. :ill. (some col.) ; : 26 cm.;
Subject:	Microscopy, Fluorescence. -
Online resource:	An electronic book accessible through the World Wide Web; click for information
ISBN:	9780195177206

Molecular imaging = FRET microscopy and spectroscopy /
Molecular imagingFRET microscopy and spectroscopy /[electronic resource] :edited by Ammasi Periasamy, Richard N. Day. - Oxford ;Published for the American Physiological Society by Oxford University Press,2005. - xv, 312 p. :ill. (some col.) ;26 cm. - The American Physiological Society methods in physiology series. - Methods in physiology series..

Includes bibliographical references and index.

Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Electronic reproduction.
Amsterdam :
Elsevier Science & Technology,
2007.

Mode of access: World Wide Web.

ISBN: 9780195177206

Source: 137033:137168Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

255712
Microscopy, Fluorescence.
Index Terms--Genre/Form:

96803
Electronic books.

LC Class. No.: QH212.F55 / M64 2005eb

Dewey Class. No.: 570/.28

National Agricultural Library Call No.: QH212.F55 / M64 2005

Molecular imaging = FRET microscopy and spectroscopy /
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245 0 0 $a Molecular imaging $h [electronic resource] : $b FRET microscopy and spectroscopy / $c edited by Ammasi Periasamy, Richard N. Day.
260 $a Oxford ; $a New York : $c 2005. $b Published for the American Physiological Society by Oxford University Press,
300 $a xv, 312 p. : $b ill. (some col.) ; $c 26 cm.
490 1 $a The American Physiological Society methods in physiology series
504 $a Includes bibliographical references and index.
505 0 $a Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy - Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization;Bioluminescence RET (BRET): Techniques and Potential; Quantifying Molecular Interactions with Fluorescence Correlation Spectrosocpy; Mapping Molecular Interactions and Transport in Cell Membranes by Image Correlation Spectroscopy; Time-Correlated Single Photon Counting (TCSPC) FLIM-FRET Microscopy for Protein Localization.
520 $a The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided. The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.
533 $a Electronic reproduction. $b Amsterdam : $c Elsevier Science & Technology, $d 2007. $n Mode of access: World Wide Web. $n System requirements: Web browser. $n Title from title screen (viewed on July 25, 2007). $n Access may be restricted to users at subscribing institutions.
650 2 $a Microscopy, Fluorescence. $3 255712
650 2 $a Microscopy, Fluorescence $x methods. $3 96468
650 2 $a Spectrometry, Fluorescence. $3 319731
650 2 $a Spectrometry, Fluorescence $x methods. $3 319732
650 2 $a Fluorescence Resonance Energy Transfer. $3 319733
650 6 $a Microscopie de fluorescence. $3 319734
650 6 $a Spectroscopie de fluorescence. $3 319735
650 0 $a Fluorescence microscopy. $3 97640
650 0 $a Fluorescence spectroscopy. $3 120907
655 7 $a Electronic books. $2 local. $3 96803
700 1 $a Periasamy, Ammasi. $3 265463
700 1 $a Day, Richard N. $3 319730
710 2 $a American Physiological Society (1887- ) $3 319729
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776 1 $c Original $z 0195177207 $z 9780195177206 $w (DLC) 2004061702 $w (OCoLC)57406048
830 0 $a Methods in physiology series. $3 319728
856 4 0 $3 ScienceDirect $u http://www.sciencedirect.com/science/book/9780195177206 $z An electronic book accessible through the World Wide Web; click for information
994 $a C0 $b TEF